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US 12,498,379 B2Granted
Solid-phase N-terminal peptide capture and release
AssigneeBoard of Regents, The University of Texas System
InventorsEric Anslyn, Edward Marcotte, Cecil J. Howard, II, Jagannath Swaminathan, Angela M. Bardo, Brendan M. Floyd, James Reuther
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Abstract
Provided herein are rapid and reversible methods to non-specifically immobilize peptides and proteins irrespective of their sequence, as well as small molecules, on a solid support to allow for manipulations of and reactions with these molecules in a manner that does not require purification between steps, which increases sample yield and reduces the quantity of starting material required.
Claims6
A conjugating group selected from the group consisting of:
- wherein the aromatic or heteroaromatic ring of the above formulas is optionally substituted with one or more functional groups, each independently selected from the group consisting of amino, cyano, halo, hydroxy, nitro, and a group of the formula:
- —[N(Ra)(Rb)(Rc)]*, wherein Ra, Rb, and Rc are each independently selected from the group consisting of hydrogen and optionally substituted C1-C8 alkyl;
- represents an attachment point to a solid support;
- and
- R comprises a Rink linker and/or a polyethylene glycol linker.
2 more elements
Dependencies are inferred from the claim text.