Combined invasion and cytotoxicity assay using chemokine secreting target cells
AssigneeImmunityBio, Inc.
InventorsNathan Thomas Schomer, Laurent H. Boissel, Hans G. Klingemann
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Abstract
Provided herein are compositions and methods for detecting migration of effector cells towards a target cell, and cytotoxicity of the migrated effector cells against the target cells. The effector cells are modified to express a homing or migratory receptor, and the target cells are modified to express the cognate ligand. The methods can be carried out in a Boyden chamber or tranwells with a porous membrane between the wells. The membrane can be coated with an extracellular matrix component to simulate a solid tumor environment.
Claims13
A method for detecting migration and cytotoxicity of effector cells, the method comprising:
- providing a device comprising two or more chambers, wherein at least a first chamber is separated by a membrane from a second chamber, the membrane comprising pores having a diameter that permits cells to pass through the membrane;
- adding effector cells to the first chamber, wherein the effector cell is modified to express a homing receptor, wherein the homing receptor is CCR7;
- adding target cells to the second chamber, wherein the target cell is modified to express a ligand that binds to the homing receptor;
- allowing the effector cells to migrate from the first chamber to the second chamber;
- and
- measuring the number of target cells killed by the effector cells in the second chamber, wherein the effector cells are NK-92 cells.
Dependencies are inferred from the claim text.