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US 12,498,362 B2Granted
Method for examining cancer in pet animal
AssigneeHirotsu Bio Science Inc.
InventorsTakaaki Hirotsu, Satoru Kaifuchi, Toshimi Sugimoto, Hideshi Ishii, Masamitsu Konno, Yuji Ueda, Shogo Kobayashi, Hidetoshi Eguchi, Yuichiro Doki, Masaki Mori
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Abstract
A method of examining for cancer in a non-primate mammal such as a pet animal may include, for example, a method of examining or diagnosing for cancer in a non-primate mammal, including evaluating taxic behavior of nematodes toward a urine sample from a subject mammal.
Claims14
A method of diagnosing cancer in a dog or cat, the method comprising:
- providing a urine sample from a dog or from a cat;
- diluting the urine sample in a solvent to provide a first diluted urine sample having a first dilution factor;
- diluting the urine sample in a solvent to provide a second diluted urine sample having a second dilution factor;
- placing N2 Bristol strain nematodes at a predetermined distance from each of the first diluted urine sample and the second diluted urine sample
- assaying attraction of nematodes to the first and second diluted urine samples;
- and
- detecting attraction of nematodes to at least one of the first and second diluted urine samples, thereby diagnosing cancer,
- wherein the cancer is a liver tumor or angiosarcoma when the urine sample is
- from a dog and wherein the cancer is a lung tumor, breast tumor, intranasal glandular tumor or lymphoma when the urine sample is from a cat;
- wherein the first and second dilution factors are different and are each independently selected from a dilution factor that is from 1×10−2 to 1×10−3.
7 more elements
Dependencies are inferred from the claim text.