Microscopic confocal fluorescence spectrometer

A microscopic confocal fluorescence spectrometer, comprising:

• an optical system, the optical system comprises a laser, a laser filter, a nanoparticle film linear polarizer, a laser attenuation device, an edge filter switching device, a dichroic mirror switching device, a beam splitter switching device, a video switching device, a confocal hole, a grating switching and rotating device, a spectrum detection device, a triaxial sample object stage and an objective lens;
• a light beam emitted by the laser is filtered by the laser filter and reflected, the reflected light beam is incident into the nanoparticle film linear polarizer, a light beam emitted from the nanoparticle film linear polarizer is attenuated by the laser attenuation device, the attenuated light beam is incident into an edge filter group of the edge filter switching device, the light beam reflected by the edge filter switching device passes through the dichroic mirror switching device, the beam splitter switching device and the video switching device in sequence, the light beam reflected by the video switching device irradiates on a measured substance through the objective lens, a fluorescence signal and scattered light produced by the measured substance after excitation are collected by the objective lens, reflected by the video switching device, and transmitted through the beam splitter switching device and the dichroic mirror switching device in sequence to reach the edge filter switching device;
• the scattered light is filtered by the edge filter switching device, the fluorescence signal is focused on the confocal hole and irradiates on a grating in the grating switching and rotating device for light splitting, and focused on to the spectrum detection device to form a fluorescence spectrum;
• the triaxial sample object stage is capable of carrying the measured substance and driving the measured substance to move along a horizontal plane and a vertical plane, and the objective lens is arranged directly facing the triaxial sample object stage;
• an instrument body, the instrument body comprises an instrument base plate, and a first shell and a second shell which are arranged on the instrument base plate;
• the first shell and the second shell are arranged side by side, sum of base areas of the first shell and the second shell is smaller than top surface area of the instrument base plate, and the first shell comprises a suspended structure;
• the laser, the laser filter, the nanoparticle film linear polarizer, the laser attenuation device, the edge filter switching device and the video switching device are all arranged in an inner cavity of the first shell, the objective lens is arranged at a bottom of the suspended structure of the first shell, and the triaxial sample object stage is arranged on the instrument base plate below the objective lens;
• the confocal hole and the grating switching and rotating device are arranged in an inner cavity of the second shell, the inner cavity of the second shell is a sealed light shielding cavity, and the spectrum detection device is arranged on an outer wall of the second shell.