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US 12,497,638 B2Granted
Methods of producing epimerases and benzylisoquinoline alkaloids
AssigneeThe Board of Trustees of the Leland Stanford Junior University
InventorsChristina D. Smolke, Stephanie Galanie, Isis Trenchard, Catherine Thodey, Yanran Li
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Abstract
A method of epimerizing an (S)-1-benzylisoquinoline alkaloid to an (R)-1-benzylisoquinoline alkaloid is provided. The method comprises contacting the (S)-1-benzylisoquinoline alkaloid with at least one enzyme. Contacting the (S)-1-benzylisoquinoline alkaloid with the at least one enzyme converts the (S)-1-benzylisoquinoline alkaloid to an (R)-1-benzylisoquinoline alkaloid.
Claims14
An engineered non-plant cell comprising:
- at least one enzyme that converts an(S)-1-benzylisoquinoline alkaloid to an (R)-1-benzylisoquinoline alkaloid, wherein the at least one enzyme comprises an epimerase having at least 85% sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NOs:
- 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, and 15;
- and
- wherein the(S)-1-benzylisoquinoline alkaloid is epimerized to an (R)-1-benzylisoquinoline within the engineered non-plant cell.
1 more element
Dependencies are inferred from the claim text.