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US 12,497,611 B2Granted
Compositions and methods for high-throughput activation screening to boost T cell effector function
AssigneeYale University
InventorsSidi Chen, Ryan D. Chow, Lupeng Ye, Matthew Dong, Jonathan Park, Lei Peng
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Abstract
The present invention includes dead-guide RNA (dgRNA) libraries and methods of use in immunology and immunotherapy thereof. Also provided are engineered primary or chimeric antigen receptor (CAR) T cells that overexpress Prodh2, Ccnblip1, Sreklip1, or Wdr37 or a fragment thereof, and methods of use in immunology and immunotherapy thereof.
Claims19
A vector comprising
- (i) a first expression cassette comprising a nucleic acid sequence encoding a dead-guide RNA (dgRNA), and a MS2-loop-containing chimeric backbone sequence, under control of a first promoter, and
- (ii) a second expression cassette comprising a nucleic acid sequence encoding a fusion protein of Thy1.1 and one or more transcription activators under control of a second promoter,
- wherein the dgRNA consists of a nucleotide sequence selected from the group consisting of any one of SEQ ID NOs:
- 1-83600 and 83601.
1 more element
A vector comprising
- (i) a first expression cassette under control of a first promoter, comprising a dead-guide RNA (dgRNA) and a MS2-loop-containing chimeric backbone sequence, and
- (ii) a second expression cassette under control of a second promoter, comprising a fusion protein of Thy1.1 and one or more transcription activators, wherein the vector comprises a nucleic acid sequence of SEQ ID NO:
- 84602.
Dependencies are inferred from the claim text.