Method of purifying whole virus particles
AssigneeHvidovre Hospital
InventorsJudith Margarete Gottwein, Jens Bukh, Anna Offersgaard, Anne Finne Pihl, Michael Wolff, Keven Lothert
Life of this patent
Estimating the term…
Abstract
The present invention relates to a method of purifying whole HCV particles, the method comprising the steps of a) providing a cell culture supernatant comprising virus particles, b) purification and/or concentration of the cell culture supernatant, c) purification and/or concentration of the product of above step b) using steric exclusion chromatography (SXC) at alkaline pH in the range of 8-10, d) purification and/or concentration of the product of the above step c) using sulphated cellulose membrane absorbers (SCMA), e) obtaining whole virus particles.
Claims15
A method of purifying whole hepatitis C virus (HCV) particles, the method comprising:
- a) providing a cell culture supernatant comprising virus particles,
- b) purifying and/or concentrating the cell culture supernatant,
- c) purifying and/or concentrating the product of step b) using steric exclusion chromatography (SXC) at alkaline pH in the range of 8-10,
- d) purifying and/or concentrating the product of step c) using sulphated cellulose membrane absorbers (SCMA), and
- e) obtaining purified whole virus particles.
Dependencies are inferred from the claim text.