Bioreactor for cell culture

A bioreactor for cell culture, comprising:

• an upper open-type container in which a cell culture medium (or solution) is accommodated;
• a pedestal seated on a top of the container, which is sealed with a circular seal between the top of the container and the pedestal and has an opening hole formed in the center thereof;
• a head plate which is seated and mounted on the pedestal to block the opening hole of the pedestal, and is provided with a shaft hole in the center thereof;
• a stirring motor fixed on the head plate;
• a hollow stirring shaft connected to the stirring motor, which is immersed in the cell culture solution;
• a stirring blade mounted on the stirring shaft, which rotates together with the stirring shaft and agitates the cell culture solution, and includes an inner vibrating plate to transmit vibration generated by an ultrasonic generator to the cell culture solution around the stirring shaft, thereby inducing cell separation;
• and
• an outer vibrating plate mounted on an outer surface of the container, which transmits the vibration generated from a second ultrasonic generator to the cell culture solution through the container wherein the vibration is transmitted to the cell culture solution near an inner peripheral surface of the container to separate the cells;
• a built-in type cell culture module mounted between the head plate and the stirring blade inside the container, which enables the cells to be adhered and cultured;
• a hollow tube installed to be spaced apart from an outer peripheral surface of the stirring shaft, in which an upper end is fixed to the head plate;
• a perforated bin provided integrally on an outer peripheral surface of the hollow tube, in which perforated holes are formed to circulate the cell culture solution throughout the entire surface;
• at least one or more spiral tubes disposed to connect between upper and lower plates of the perforated bin, in which perforated holes are formed to circulate the cell culture solution;
• an ultrasonic vibrator mounted on a coupling site with the head plate of the hollow tube, and
• wherein the bioreactor further includes an outlet provided at an upper side of the perforated bin in the hollow tube in order to discharge a water flow, which rises from a lower side to an upper side in the hollow tube by the stirring blade, to the outside of the tube.