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US 12,497,443 B2Granted
Systems and methods for the production of human polyclonal antibodies
AssigneeSab, LLC
InventorsJay Hooper, Eddie Sullivan, Hua Wu
Life of this patent
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Abstract
Disclosed herein is a method for producing human antibodies against a pathogen comprising injecting a non-human animal with a pathogen-derived DNA vaccine in at least two locations of the animal; injecting the animal with an adjuvant in a location of the animal different from the location of the DNA vaccine location; collecting plasma from the animal after the injections; and purifying polyclonal antibody from the plasma.
Claims16
A method for producing human antibodies against a DNA vaccine, comprising:
- a) administering a transchromosomic ungulate with at least two DNA vaccines in at least two separate locations of the animal transchromosomic ungulate, the at least two DNA vaccines comprise:
- i) a first DNA vaccine encoding a first antigen, and
- ii) a second DNA vaccine encoding a second antigen, wherein the first antigen is injected at a first location and the second antigen is injected at a second location, wherein the first antigen and the second antigen are for the same disease target;
- and wherein the transchromosomic ungulate produces human IgG antibodies, wherein the transchromosomic ungulate is a knock-out for endogenous ungulate immunoglobulin genes, and comprises a human artificial chromosome (HAC) vector containing the full germ line sequence of human immunoglobulins;
- b) collecting plasma from the transchromosomic ungulate;
- and
- c) purifying polyclonal human IgG antibodies from the plasma.
4 more elements
Dependencies are inferred from the claim text.