Polymer-hydroxyapatite composite membranes
AssigneeBio-Rad Laboratories, Inc.
InventorsShawn Darnall, Brian Panganiban, Danni Wang, Christopher Belisle, Zachary Clauss
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Abstract
Provided herein is a chromatography media composed of a porous solid substrate (such as a membrane, metal, or metallic alloy) that is coated with hydroxyapatite (HA). Also provided is a chromatography media comprising a HA-coated substrate and uses thereof. Thus this disclosure provides a chromatography media composed of a porous solid substrate (such as a membrane, metal, or metallic alloy) that is coated with HA. Also provided is a chromatography media comprising a HA-coated substrate and uses thereof. Methods of preparing the HA-coated substrate are also provided.
Claims23
A hydroxyapatite (HA)-coated substrate made by a method comprising:
- a) contacting a solid porous substrate comprising a hydroxyl group, amine group, quaternary amine group, aldehyde group, azide group, alkyne group, alkene group, phosphate group, sulfonate group, sulfate group, and/or carboxylic acid group with a solution comprising calcium ions, with the proviso that if a substrate comprises positively charged groups it is contacted with a phosphate containing solution and washed prior to contact with a solution comprising calcium ions;
- b) washing the solid substrate contacted with the solution comprising calcium ions with a wash solution;
- c) contacting the washed solid substrate of step b) with a solution comprising phosphate ions to form a solid substrate coated with a brushite form of calcium phosphate;
- d) washing the solid substrate of step c) with a solution to form a brushite-coated substrate;
- and
- e) optionally contacting the solid substrate of step d) with a solution comprising calcium ions and repeating steps b), c), and d);
- and
- f) treating the brushite coated substrate with a base to form a HA-coated substrate, with the proviso that the substrate is not a particulate substrate;
- wherein the solid porous substrate comprises:
- i) cellulose, nitrocellulose, nylon, glass, methacrylate, polystyrene, polyethersulfone, or mixtures thereof;
- or
- ii) glass, metal, a metallic alloy, nylon, polystyrene, cellulose, polyethersulfone, or nitrocellulose.
A hydroxyapatite (HA)-coated substrate made by a method comprising:
- a) contacting a solid porous substrate comprising positively charged groups with a solution comprising phosphate ions;
- b) washing the solid porous substrate contacted with the solution comprising phosphate ions;
- c) contacting the solid porous substrate of step b) with a solution comprising calcium ions;
- d) washing the solid substrate contacted with the solution comprising calcium ions with a wash solution;
- e) contacting the washed solid substrate of step b) with a solution comprising phosphate ions to form a solid substrate coated with a brushite form of calcium phosphate;
- f) washing the solid substrate of step c) with a solution to form a brushite-coated substrate;
- and
- g) optionally repeating steps c), d), e), and f);
- and
- h) treating the brushite coated substrate with a base to form a HA-coated substrate, with the proviso that the substrate is not a particulate substrate.
A method of making a hydroxyapatite (HA)-coated substrate comprising:
- a) contacting a solid porous substrate comprising a hydroxyl group, amine group, quaternary amine group, aldehyde group, azide group, alkyne group, alkene group, phosphate group, sulfonate group, sulfate group, and/or carboxylic acid group with a solution comprising calcium ions;
- b) washing the solid substrate contacted with solution of calcium ions with a wash solution;
- c) contacting the washed solid substrate of step b) with a solution comprising phosphate ions to form a solid substrate coated with a brushite form of calcium phosphate;
- d) washing the solid substrate of step c) with a solution to form a brushite-coated substrate;
- and
- e) optionally repeating steps b), c), and d);
- and
- f) treating the brushite coated substrate with a base to form a HA-coated substrate, with the proviso that the substrate is not a particulate substrate.
Dependencies are inferred from the claim text.