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US 12,495,792 B2Granted
Cell sheet protection solution
AssigneeBeijing BOE Technology Development Co., Ltd.
InventorsJuan Wang, Yufei Zhao, Shuang Gao, Xin Jin, Yuqin Tan, Zhisheng Li, Dehua Chang
Life of this patent
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Abstract
The present disclosure relates to a cell sheet protection solution, such as a stem cell sheet protection solution, such as a mesenchymal stem cell sheet protection solution. The present disclosure also relates to use of the cell sheet protection solution in storage and transportation of the cell sheet.
Claims8
A cell sheet protection solution comprising the following components:
- a. a buffered salt solution;
- b. an extracellular matrix component;
- c. a reducing agent;
- d. a substance required for cell metabolism;
- and
- e. a hormone
- wherein the buffered salt solution is phosphate buffered saline (PBS), the extracellular matrix component includes gelatin, the concentration of gelatin being 0.5-5 mg/L:
- the reducing agent includes vitamin C and glutathione, the concentration of vitamin C being 100-1000 mg/L and the concentration of glutathione being 5-30 mg/L:
- the substance required for cell metabolism includes transferrin, linoleic acid and linolenic acid, the concentration of transferrin being 5-30 mg/L, the concentration of linoleic acid being 0.5-5 mg/L and the concentration of linolenic acid being 6.5-5 mg/L;
- and the hormone includes insulin, the concentration of insulin being 5-30 mg/L.
7 more elements
Dependencies are inferred from the claim text.