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US 12,495,790 B2Granted
Use of polyelectrolytes as cryoprotectants and a method of cryopreservation with their use
AssigneeUniwersytet Jagiellonski
InventorsMaria Nowakowska, Ewa Zuba-Surma, Krzysztof Szczubialka, Mateusz Zajac, Kamil Kaminski, Elzbieta Karnas, Katarzyna Kmiotek-Wasylewska
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Abstract
The subject of the invention is the use of selected polyelectrolytes from the group of synthetic and natural polyelectrolytes for the cryopreservation of human and animal extracellular vesicles. The invention also relates to a method for the cryopreservation of these vesicles, which uses said poly electrolytes.
Claims5
A method of cryopreservation of extracellular vesicles comprising the following steps:
- a) isolating extracellular vesicles;
- b) coating with a layer of a first polyelectrolyte, wherein the first polyelectrolyte is a block copolymer selected from copolymers represented by the general formulas:
- PEGx-b-PMAPTACy,
- wherein PEG is polyethylene qlycol and PMAPTAC is poly((3-(methacryloylamino)propyl)trimethylammonium chloride), x and y are degrees of polymerization in the range from 10 to 45 and from 5 to 102, respectively;
- and “b” denotes “block”;
- c) coating with a layer of a second polyelectrolyte, wherein the second polyelectrolyte is an anionic homopolymer selected from polymers represented by the general formula:
- PAMPSx
- wherein PAMPS is poly(2-acrylamido-2-methyl-1-propanesulfonic acid and x is a degree of polymerization ranging from 15 to 150;
- and
- d) freezing of the vesicles coated with the first and second layers of polyelectrolyte.
7 more elements
Dependencies are inferred from the claim text.