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US 12,493,040 B2Granted
Cancer detection method using sense of smell of nematode
AssigneeHirotsu Bio Science Inc.
InventorsTakaaki Hirotsu, Hideto Sonoda
Life of this patent
Estimating the term…
Abstract
A cancer detection method characterised in that a nematode is bred in the presence of bio-related material originating from a test subject, or a processed product of same, and cancer is detected using the chemotaxis due to the sense of smell of the nematode as an indicator.
Claims10
A method for detecting cancer in a subject, comprising the steps of:
- (a) obtaining a test sample which comprises urine, a cell culture medium, or a preservative solution of the cells or tissues from the subject and wherein a concentration of the urine, the cell culture medium, or the preservative solution of the cells or tissues is diluted with a diluting liquid, wherein the urine is diluted to 1.5 to 1000 times or the cell culture medium is diluted to 106 to 107 times, or the preservative solution of the cells or preservative solution of the tissues is diluted to 102 to 104 times;
- (b) exposing nematodes to the test sample and a control sample separately, and
- (c) assaying a chemotaxis of the nematodes to the test sample and the control sample separately,
- wherein a positive chemotaxis by the nematodes to the test sample indicates that the subject is determined to have cancer, or to have a risk of having cancer, and
- wherein a negative chemotaxis by the nematodes to the test sample indicates that the subject is not determined to have cancer, or have the risk of having cancer, wherein the nematodes are a member of the genus Caenorhabditis.
2 more elements
A method for detecting cancer in a subject, comprising the steps of:
- (a) obtaining a test sample which comprises urine, a cell culture medium, or a preservative solution of the cells or tissues from the subject and wherein a concentration of the urine, the cell culture medium, or the preservative solution of the cells or tissues is diluted with a diluting liquid, wherein the urine is diluted to 1.5 to 1000 times or the cell culture medium is diluted to 106 to 107 times, or the preservative solution of the cells or preservative solution of the tissues is diluted to 102 to 104 times;
- (b) placing separately nematodes and the test sample on an assay plate to induce chemotaxis behavior of the nematodes,
- (c) assaying a chemotaxis of the nematodes to the test sample,
- wherein a positive chemotaxis by the nematodes to the test sample indicates that the subject is determined to have cancer, or to have a risk of having cancer, and
- wherein a negative chemotaxis by the nematodes to the test sample indicates that the subject is not determined to have cancer, or have the risk of having cancer, wherein the nematodes are a member of the genus Caenorhabditis.
2 more elements
Dependencies are inferred from the claim text.