Methods for forming a matrix for receiving tissue samples
AssigneeLeavitt Medical, Inc.
InventorSorin Musat
Life of this patent
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Abstract
A custom-made matrix suitable for receiving a tissue sample is described, as well as the use thereof to obtain a multiplex histological preparation. The disclosure also relates to a multiplex biopsy array comprising tissue and/or cell samples arranged in a matrix material and to a method for the preparation of a multiplex biopsy array. Methods for preparing blocks of matrix material to be used in multiplex biopsy arrays are also described, as well as methods for loading biopsy samples in the blocks, and methods for treating and processing the blocks to form biopsy arrays. The biopsy arrays made using the block of matrix material can be used to prepare sections and slides for histological procedures, including quantitative analyses and parallel processing.
Claims14
A method for forming a matrix for receiving tissue samples, the method comprising:
- forming a mixture by combining 2-14% w/v protein, 2.1-20% w/v one or more lipids selected from the group consisting of animal fat and vegetable oil, 2-15% w/v glycerin, water, a gelling agent, and an inorganic buffer in a concentration in the range of from 10 to 600 mM;
- gelling the mixture to form a matrix;
- and
- forming at least one receptacle in the matrix, the at least one receptacle shaped and sized to receive at least one tissue sample therein,
- wherein the matrix is configured to be microtome sectionable.
Dependencies are inferred from the claim text.