Fabry perot interferometry for measuring cell viability
AssigneeNirrin Technologies, Inc.
InventorsBryan A. Hassell, Walid A. Atia, David P. Marchessault
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Abstract
A method for studying cell viability and protein aggregation involves establishing a Fabry Perot etalon signal within an optical spectroscopic feature, e.g., in the near infrared region. Protein aggregation and cell viability can be reflected by changes observed in the magnitude of the Fourier Transform peaks observed in the frequency or space domain associated with the contrast of the etalon. In short, the presence of viable cells and protein aggregates can degrade the etalon contrast of an etalon window. In some cases, the concentration of cells and monomeric protein can be measured as well.
Claims19
A bioreactor system for analyzing for structures in a solution, the system comprising:
- a bioreactor providing cells in a culture medium;
- a flow cell containing the cells in the culture medium;
- a light source for illuminating the cells in the culture medium across a spectral band;
- a detector for detecting light from the light source after interaction with the cells in the culture medium and an etalon;
- and
- a controller configured to analyze a response of the detector to resolve contrast information of the etalon to assess the cells in the culture medium.
A bioreactor system for analyzing structures in a medium, the system comprising:
- a bioreactor for protein preparation or purification;
- a flow cell containing protein aggregates in a medium;
- a light source for illuminating the protein aggregates in the medium across a spectral band;
- a detector for detecting light from the light source after interaction with the protein aggregates in the medium and an etalon;
- and
- a controller configured to analyze a response of the detector to resolve contrast information of the etalon to assess the protein aggregates in the medium.
Dependencies are inferred from the claim text.