Metal-binding protein and use thereof
AssigneeThe Penn State Research Foundation
InventorsJoseph A. Cotruvo, Jr., Joseph A. Mattocks
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Abstract
A metal-binding protein having a high selective affinity for certain trivalent cations and/or tetravalent cations derived from, for example, rare earth elements or ions thereof such as lanthanide elements and actinides or ions thereof as well as from hafnium and zirconium elements or compounds thereof is disclosed as well as sensors including same and methods for capturing and separating such trivalent cations and/or tetravalent cations using the metal-binding protein.
Claims18
An isolated metal-binding protein comprising (i) a sequence of SEQ ID NO:41 or (ii) a sequence having at least 80% identity to SEQ ID NO:41,
- wherein the isolated metal-binding protein is immobilized on a substrate;
- wherein the isolated metal-binding protein has an apparent Kd value for a trivalent rare earth element at least 10,000-fold smaller than its apparent Kd value for calcium.
An isolated metal-binding protein comprising (i) a sequence of SEQ ID NO:41 or (ii) a sequence having at least 80% identity to SEQ ID NO:41,
- wherein the isolated metal-binding protein is immobilized on a substrate;
- wherein the isolated metal-binding protein has an apparent Kd value for a trivalent rare earth element that is at least 10,000 fold smaller than its apparent Kd value for calcium;
- wherein the isolated metal-binding protein comprising (ii) comprises two or more metal-binding motifs and at least one of the metal-binding motifs comprises at least 3 carboxylate residues, and wherein adjacent metal-binding motifs are separated by 10−15 amino acid residues.
Dependencies are inferred from the claim text.