Sex identification specific DNA sequence and sex identification method of chinese mitten crab Eriocheir sinensis

Abstract

Disclosed is a sex identification specific DNA sequence and a sex identification method of Chinese mitten crab Eriocheir sinensis. The sex identification specific DNA sequence is showed as SEQ ID NO:1. The sex identification method includes following steps: S1, extracting genomic DNA of Chinese mitten crab Eriocheir sinensis; S2, PCR amplification using the genomic DNA as template; an upstream primer for PCR amplification is as shown in SEQ ID No: 2 and a downstream primer is as shown in SEQ ID No: 3; S3, examining amplified products by agarose electrophoresis, and those with specific amplified bands are females, otherwise they are males. The method has high identification accuracy, is simple and easy to operate, and is suitable for sex identification of Chinese mitten crab Eriocheir sinensis at embryonic and larval stages.

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