Devices and methods for detecting microorganisms using recombinant reproduction-deficient indicator bacteriophage
AssigneeLaboratory Corporation of America Holdings
InventorsStephen Erickson, Jose S. Gil, Minh Mindy Bao Nguyen, Wendy S. Hahn
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Abstract
Disclosed herein are compositions, methods, kits and systems for rapid detection of microorganisms using a reproduction-deficient indicator bacteriophage. The specificity of such reproduction-deficient indicator bacteriophage for binding and infecting particular microorganisms of interest allows targeted and sensitive detection of a microorganism of interest.
Claims20
A method of detecting at least two microorganisms of interest in a sample, comprising:
- incubating a sample with at least two recombinant phages, each comprising an indicator gene in a late gene region of genome of the phage, wherein the recombinant phages are reproduction-deficient, and wherein the recombinant phages are capable of specifically infecting one or more microorganism of interest;
- and,
- detecting products of the indicator genes, wherein positive detection of the products of the indicator genes indicates that the microorganisms of interest are present in the sample.
A kit for detecting at least two microorganisms of interest in a sample comprising:
- at least two recombinant phages, each comprising an indicator gene in a late gene region of genome of the phage, wherein the recombinant phages are reproduction-deficient, and wherein the recombinant phages are capable of specifically infecting one or more microorganism of interest;
- and
- a substrate for reacting with products of the indicator genes to detect the products of the indicator genes.
A system for detecting at least two microorganisms of interest comprising:
- at least two recombinant phages, each comprising an indicator gene in a late gene region of genome of the phage, wherein the recombinant phages are reproduction-deficient, and wherein the recombinant phages are capable of specifically infecting one or more microorganism of interest;
- and
- components for detecting the products of the indicator genes.
Dependencies are inferred from the claim text.