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US 12,492,420 B2Granted
Compositions, kits, and methods for in vitro transcription
OwnerDillon B. Nye, Jennifer L. Curcuru +3inventors
InventorsDillon B. Nye, Jennifer L. Curcuru, Tien-Hao Chen, Lili Mitchell, Ivan R. Correa, Jr.
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Abstract
The present disclosure relates, according to some embodiments, to cold-active RNA polymerases, variants thereof, compositions and kits comprising cold-active RNA polymerases, and methods of using cold-active RNA polymerases. Cold-active polymerases may have, for example, an amino acid sequence ≥90%, ≥91%, ≥92%, ≥93%, ≥94%, ≥95%, ≥96%, ≥97%, ≥98%, ≥99% identical (e.g., ≥95% or ≥98% identical) to any of SEQ ID NOS: 1-19 and optionally may have at least one substitution relative to SEQ ID NO:1.
Claims16
A composition comprising:
- a cold-active RNA polymerase having an amino acid sequence at least 95% identical to any of SEQ ID NOS:
- 1, 5, 6, 8-10, 12, 13, 18, and 19;
- and
- a capping enzyme,
- wherein the cold-active RNA polymerase is a non-naturally occurring cold-active RNA polymerase or a cold-active RNA polymerase of Pseudomonas phage Njord, Pseudomonas phage uligo, Vibrio phage φA318, Vibrio phage Vp670, Vibrio phage Vc1, Vibrio phage VEN, and the capping enzyme is a non-naturally occurring capping enzyme or a capping enzyme of Faustovirus, mimivirus, or moumouvirus.
2 more elements
Dependencies are inferred from the claim text.