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US 12,492,416 B2Granted
Production of L-2-aminobutyrate from citramalate, citraconate or 2-oxobutanoate
AssigneeAnna University
InventorsAkila Thirumalai, Padmapriya Ganapathy Raman, Anitha Janet Roshni Yesudhas, Tamilselvan Jayavelu, Nikhil Sangith, Mahalakshmi Natarajan, Pandiraj Suppuram, Ramalingam Subramanian
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Abstract
The present invention relates to preparation of key drug intermediate, L-2-amino butyric acid (L-2-ABA) by a method of cell free system and biotransformation using genetically engineered strains from easily available economic substrates like citramalate or citraconate and enzymes like LeuCD, LeuB and ValDH or IlvE.
Claims3
A process for production of L-2-Aminobutyric acid in a cell free system, comprising:
- a) transforming a microbial host cell to incorporate genes leu CD, leu B, and valDH to express enzymes being an isopropyl malate isomerase, an isopropylmalate dehydrogenase and a valine dehydrogenase;
- b) harvesting and lysing of the host cell to obtain a crude lysate comprising the isopropyl malate isomerase, the isopropylmalate dehydrogenase and the valine dehydrogenase;
- c) optionally isopurifying from the crude lysate the isopropyl malate isomerase, the isopropylmalate dehydrogenase and the valine dehydrogenase to produce isolated enzymes, or optionally immobilizing from the crude lysate the isopropyl malate isomerase, the isopropylmalate dehydrogenase and the valine dehydrogenase to produce immobilized enzymes;
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- d) contacting in a cell free system having a pH of 7 to 9 a substrate being citramalate or citraconate with the crude lysate, purified isolated enzymes or the immobilized enzymes to produce L-2-Aminobutyric acid.
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Dependencies are inferred from the claim text.