Genetically modified phototrophic cell for in-vivo production of hydrogen
AssigneeUniversity of Kassel
InventorsKirstin Gutekunst, Jens Appel
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Abstract
A genetically modified phototrophic cell for in-vivo production of hydrogen. The phototrophic cell has been genetically modified to the effect that a) at least one of the native photosystem I components has been deleted, b) the native hydrogenase has been deleted, and c) at least one fusion protein is expressed, comprising i. a hydrogenase or hydrogenase component and ii. at least one PSI component, with the proviso that the PSI is complemented by expression of the at least one fusion protein, and the hydrogenase component itself, or together with at least one further hydrogenase component expressibly introduced into the cell, has hydrogenase activity.
Claims15
A phototrophic cell, which in the wild type has a native photosystem I, PSI, with PSI components and a native hydrogenase, wherein the phototrophic cell has been genetically modified to the extent that
- a) at least one of the native PSI components has been deleted,
- b) the native hydrogenase has been deleted, and
- c) at least one fusion protein is expressed which comprises i. a hydrogenase or hydrogenase component and ii. at least one PSI component, the PSI component being a PSI subunit or a N-terminally or C-terminally truncated PSI subunit, with the proviso that the PSI is complemented by expression of the at least one fusion protein and the hydrogenase component exhibits hydrogenase activity alone or together with at least one further hydrogenase component which has been expressibly introduced into the cell.
Dependencies are inferred from the claim text.