Methods of purifying recombinant ADAMTS13 and other proteins and compositions thereof

An in vitro method for purifying a composition comprising ADAMTS13, said method comprising:

• providing a solution comprising ADAMTS13;
• immobilizing said ADAMTS13 on a cation exchange resin, wherein said immobilization step is performed in the absence of a solvent-detergent mixture;
• and
• subsequently incubating said immobilized ADAMTS13, with a solvent-detergent mixture at concentrations and for a time period suitable to inactivate a lipid-enveloped virus, wherein said solvent-detergent mixture comprises a non-ionic detergent and an organic solvent and
• wherein said method results in a reduction in formation of aggregates of said ADAMTS13 as compared to a comparable method, wherein said comparable method comprises incubation with said solvent-detergent mixture while said ADAMTS13 is not immobilized.

An in vitro method for inactivating a lipid-enveloped virus contaminant in a protein solution during the purification of a therapeutic protein composition, said method comprising:

• providing the solution comprising the therapeutic protein;
• immobilizing said therapeutic protein on an anion exchange resin or cation exchange resin, wherein said immobilization step is performed in the absence of a solvent-detergent mixture;
• and
• subsequently incubating said immobilized therapeutic protein, with a solvent-detergent mixture at concentrations and for a time period suitable to inactivate the lipid-enveloped virus contaminant, wherein said solvent-detergent mixture comprises a non-ionic detergent and an organic solvent and
• wherein said method results in a reduction in formation of aggregates of said therapeutic protein as compared to a comparable method, wherein said comparable method comprises incubation with said solvent-detergent mixture while said therapeutic protein is not immobilized.