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US 12,492,374 B2Granted
Micro-engineered models of the human eye and methods of use
AssigneeThe Trustees of the University of Pennsylvania
InventorsSunghee Estelle Park, Wenli Yang, Dwight E. Stambolian, Dongeun Huh
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Abstract
This present disclosure relates to a bioengineering approach based on microphysiological culture to mimic tissue-tissue interface. Accordingly, the present disclosure provides methods, compositions and kits related to the approach.
Claims21
A microfluidic chip for three-dimensional in vitro organoid culture, the microfluidic chip comprising:
- a bottom layer comprising a center lane and at least one side microfluidic channel adjacent thereto, the at least one side microfluidic channel comprising a surface bearing endothelial cells that form a lumen within the at least one side microfluidic channel, wherein the bottom layer further comprises a cell-laden hydrogel within the center lane;
- and
- a top layer comprising a cell culture medium reservoir including retinal pigment epithelial cells, wherein the cell culture medium reservoir and the center lane are in fluidic communication.
Dependencies are inferred from the claim text.