Production method for nerve tissue

A method for producing cerebral cells or a cerebral tissue, comprising the following steps (1)-(4):

• (1) a first step of maintaining and/or expanding human pluripotent stem cells comprising culturing the human pluripotent stem cells in the absence of a feeder cell in a medium comprising a factor for maintaining an undifferentiated state of the pluripotent stem cells,
• (2) a second step of culturing the pluripotent stem cells obtained by the first step in a medium comprising (a) a Sonic hedgehog signal transduction pathway activating substance or a combination of a TGFβ family signal transduction pathway inhibiting substance and the Sonic hedgehog signal transduction pathway activating substance, and (b) a factor for maintaining an undifferentiated state of the pluripotent stem cells in the absence of feeder cells for a period of 0.5 hr-144 hr,
• (3) a third step of culturing the cells obtained in the second step in suspension to form a cell aggregate, and
• (4) a fourth step of culturing the aggregate obtained in the third step in suspension in the presence of a TGFβ family signal transduction pathway inhibiting substance and/or a Wnt signal transduction pathway inhibiting substance to obtain an aggregate containing cerebral cells or a cerebral tissue,
• wherein the factor for maintaining an undifferentiated state of the pluripotent stem cells comprises a FGF signal transduction pathway activating substance,
• wherein the FGF signal transduction pathway activating substance is bFGF,
• wherein the TGFβ family signal transduction pathway inhibiting substance is Lefty, SB431542, A-83-01 or LDN193189,
• wherein the Sonic hedgehog signal transduction pathway activating substance is Shh, SAG or Purmorphamine,
• and wherein the Wnt signal transduction pathway inhibiting substance is IWR-1-endo.