Induced stem cells
AssigneeMonash University
InventorsJose Polo, Xiaodong Liu, Jia Ping Tan
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Abstract
The present invention relates to stem cells derived from a multi-layered cellular structure or blastocyst structure, compositions comprising the same, and methods for obtaining the same.
Claims18
A human primed pluripotent stem cell (pPSC) or a cell exhibiting at least one characteristic of a pPSC, wherein the pPSC or cell exhibiting at least one characteristic of a pPSC is produced or isolated from a blastocyst-like structure, wherein the at least one characteristic of a pPSC is selected from:
- an absence of the markers characterising a somatic cell;
- expression of one or more mRNAs of epiblast specific transcription factors;
- expression of one or more of:
- SFP, OTX2, ZIC2, ZIC3, ZIC5, DNMT3B, KDR, CDH2, CER1, COL2A1, DAZL, TCF7L1, SOX11, SALL2, SOX2, NANOG, KLF4, EPCAM, PRDM14, SSEA-3, SSEA-4, TRA-1-60, TRA-1-81, TRA-2-49/6E, ALP, E-cadherin, UTF-1, OCT4 (POU5F1) and REX1;
- or
- the cell does not express one or more of the markers:
- ANPEP, TWIST2, KLF17, KRT7, ITGA2.
A method of producing a human primed pluripotent stem cell (pPSC) or a cell exhibiting at least one characteristic of a pPSC, the method comprising;
- culturing single cells dissociated from a blastocyst-like structure on a vitronectin layer in the presence of a culture medium for promoting cells to a pluripotent state,
- wherein the blastocyst-like structure is obtained by a method of aggregating reprogramming intermediates or a method of assembly of iPSCs and iTSCs,
- and wherein the at least one characteristic of a pPSC is selected from:
- an absence of the markers characterising a somatic cell;
- expression of one or more mRNAs of epiblast specific transcription factors;
- expression of one or more of:
- SFP, OTX2, ZIC2, ZIC3, ZIC5, DNMT3B, KDR, CDH2, CER1, COL2A1, DAZL, TCF7L1, SOX11, SALL2, SOX2, NANOG, KLF4, EPCAM, PRDM14, SSEA-3, SSEA-4, TRA-1-60, TRA-1-81, TRA-2-49/6E, ALP, E-cadherin, UTF-1, OCT4 (POU5F1) and REX1;
- or
- the cell does not express one or more of the markers:
- ANPEP, TWIST2, KLF17, KRT7, ITGA2,
- thereby producing the pPSC or a cell exhibiting at least one characteristic of the pPSC.
Dependencies are inferred from the claim text.