Sensor chip capable of culturing cells and of analyzing cell-derived substance, and cell activity quantitative analysis method using the same

A sensor chip for analysis of a cell-derived substance, wherein the sensor chip is capable of culturing cells, wherein the sensor chip comprises:

• a cell-culturing assembly having an upper plate having a first groove defined therein and a lower plate coupled to the upper plate and having a second groove defined therein, wherein the first and second grooves are connected to each other in a fluid-communicating manner to form a culturing space in which immune cells are cultured;
• a work electrode coupled to the upper plate so as to extend from a position out of the culturing space into the culturing space, wherein one end of the work electrode positioned inside the culturing space includes a reducing portion made of a carbon paste having horseradish peroxidase (HRP) fixed thereto;
• an auxiliary electrode coupled to the upper plate so that one end of the auxiliary electrode is positioned inside the culturing space, wherein the auxiliary electrode is spaced apart from the work electrode;
• and
• a reference electrode disposed between the upper plate and the lower plate, wherein one end of the reference electrode is positioned inside the culturing space,
• wherein the work electrode includes:
• a metal wiring,
• an insulator covering a surface of the metal wiring so as to expose one end of the metal wiring positioned inside the culturing space,
• an insulating pipe surrounding the insulator and the metal wiring, and
• the reducing portion in contact with a portion of the metal wiring not covered with the insulator, wherein the reducing portion is received in the insulating pipe.