Lipid comprising docosapentaenoic acid

A process for producing an extracted plant lipid comprising the steps of

• i) obtaining seed from at least 2,000,000 transgenic Brassica napus plants, the seed comprising lipid, the lipid comprising fatty acids in an esterified form, the fatty acids comprising oleic acid, palmitic acid, ω6 fatty acids comprising linoleic acid (LA), ω3 fatty acids comprising α-linolenic acid (ALA), docosapentaenoic acid (DPA), stearidonic acid (SDA), eicosapentaenoic acid (EPA) and eicosatetraenoic acid (ETA), wherein the level of palmitic acid in the total fatty acid content of the lipid is between 2% and 16% by weight, wherein the level of myristic acid (C14:0) in the total fatty acid content of the lipid, if present, is less than 1% by weight, wherein at least 70% of the DPA esterified in the form of triacylglycerol (TAG) is in the sn-1 or sn-3 position of the TAG, wherein the seed are transgenic for at least exogenous polynucleotides encoding a Δ12-desaturase having a sequence of amino acids provided as SEQ ID NO:
• 4, a ω3-desaturase having a sequence of amino acids provided as SEQ ID NO:
• 6, a Δ6-desaturase having a sequence of amino acids provided as SEQ ID NO:
• 9, a Δ5-desaturase having a sequence of amino acids provided as SEQ ID NO:
• 20, a Δ6-elongase having a sequence of amino acids provided as SEQ ID NO:
• 16 and a Δ5-elongase having a sequence of amino acids provided as SEQ ID NO:
• 25, and wherein each polynucleotide is operably linked to one or more promoters that are capable of directing expression of said polynucleotides in developing seed in the transgenic Brassica napus plants, and
• ii) extracting lipid from the seed.