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US 12,492,234 B2Granted
Fiber assembly and use thereof
AssigneeKyoto University
InventorAkira Kakugo
Life of this patent
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Abstract
A fiber assembly includes multiple fibers and nucleic acid fragments that crosslink the multiple fibers, and the fibers are microtubules or actin fibers.
Claims5
A method of moving a fiber assembly, comprising:
- polymerizing tubulins in the presence of guanosine-5′-triphosphate (GTP) to form soft microtubule fibers;
- associating the soft microtubule fibers with nucleic acid fragments which crosslink the fibers to form a fiber assembly, and
- bringing the fiber assembly and adenosine triphosphate (ATP) into contact with each other on a substrate surface that is covered with motor proteins,
- wherein the fiber assembly includes a first fiber to which a first single-stranded nucleic acid fragment is bound, a second fiber to which a second single-stranded nucleic acid fragment is bound, and a third single-stranded nucleic acid fragment that includes a region complementary to the first single-stranded nucleic acid fragment and a region complementary to the second single-stranded nucleic acid fragment, and
- wherein the fiber assembly moves in an arc using the chemical energy of the ATP.
2 more elements
A method of moving a fiber assembly, comprising:
- polymerizing tubulins in the presence of guanosine-5′-[α,β-methylene]triphosphate (GMPCPP) to form rigid microtubule fibers;
- associating the rigid microtubule fibers with nucleic acid fragments which crosslink the fibers to form a fiber assembly, and
- bringing the fiber assembly and adenosine triphosphate (ATP) into contact with each other on a substrate surface that is covered with motor proteins,
- wherein the fiber assembly includes a first fiber to which a first single-stranded nucleic acid fragment is bound, a second fiber to which a second single-stranded nucleic acid fragment is bound, and a third single-stranded nucleic acid fragment that includes a region complementary to the first single-stranded nucleic acid fragment and a region complementary to the second single-stranded nucleic acid fragment, and
- wherein the fiber assembly moves linearly using the chemical energy of the ATP.
2 more elements
Dependencies are inferred from the claim text.