Liquid to liquid biological particle concentrator with disposable fluid path

A system, comprising:

• a concentrating pipette tip (CPT) having:
• a housing having a top end and a bottom end;
• a filter enclosed within the housing, the filter being vertically oriented and spanning a length of the housing, the filter having a retentate surface and an opposing permeate surface;
• an elution port positioned adjacent the top end of the housing for receiving an elution fluid;
• a permeate port positioned adjacent the top end of the housing, the permeate port for coupling the housing to a vacuum source;
• and
• a sample port positioned adjacent the bottom end of the housing for aspirating a fluid sample containing a plurality of particles;
• wherein the plurality of particles in the fluid sample are tangentially eluted from the retentate surface of the filter and dispensed through the sample port;
• a first electromagnetic valve connecting the permeate port to a permeate fluid line;
• a second electromagnetic valve connecting the permeate fluid line to ambient air;
• and
• a vacuum pump creating the vacuum source and connecting the permeate fluid line to a permeate outlet.

A system, comprising:

• a concentrating pipette tip (CPT) having:
• a housing having a top end and a bottom end;
• a filter enclosed within the housing, the filter being vertically oriented and spanning a length of the housing, the filter having a retentate surface and an opposing permeate surface;
• an elution port positioned adjacent the top end of the housing for receiving an elution fluid;
• a permeate port positioned adjacent the top end of the housing, the permeate port for coupling the housing to a vacuum source;
• and
• a sample port positioned adjacent the bottom end of the housing for aspirating a fluid sample containing a plurality of particles;
• wherein the plurality of particles in the fluid sample are tangentially eluted from the retentate surface of the filter and dispensed through the sample port;
• a first electromagnetic valve connecting the permeate port to a permeate fluid line;
• a first pump creating the vacuum source and connecting the permeate fluid line to a permeate outlet;
• a second electromagnetic valve connecting the permeate port to a backpressure fluid line;
• a second pump creating backpressure;
• and
• a backpressure reservoir for storing pressure created by the second pump.