Stabilized peptide composition
AssigneeShionogi & Co., Ltd.
InventorsTakashi Murakami, Kazuyuki Takata, Yoshie Niwa, Shuichi Hatano, Hidenori Kawasaki, Satoko Fujita
Life of this patent
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Abstract
Provided herein is a peptide composition that is highly stable and can be stored long term, said composition containing one or more types of peptides having an amino acid sequence represented by any of SEQ ID Nos. 1-5, or a peptide in which one or two of the amino acids of the abovementioned peptides are independent and may be substituted, deleted or added, and saccharides and an inorganic salt.
Claims4
A method of manufacturing a lyophilized formulation, wherein the method comprises:
- a) mixing a first peptide consisting of the amino acid sequence of SEQ ID NO:
- 1, a second peptide consisting of the amino acid sequence of SEQ ID NO:
- 4, sucrose, and sodium chloride in a solution of pH 12.0 to pH 13.0;
- b) adjusting the pH of the solution prepared by said step a) to pH 10.0 to pH 12.0 with an acid substance;
- c) adding a third peptide consisting of the amino acid sequence of SEQ ID NO:
- 2, a fourth peptide consisting of the amino acid sequence of SEQ ID NO:
- 3 and a fifth peptide consisting of the amino acid sequence of SEQ ID NO:
- 5 to the solution prepared by said step b);
- d) adjusting the pH of the solution prepared by said step c) to pH 7.0 to pH 9.0 with an acid substance;
- and
- e) lyophilizing the solution prepared by said step d).
A method of manufacturing a lyophilized formulation, wherein the method comprises:
- a) mixing a first peptide consisting of the amino acid sequence of SEQ ID NO:
- 1, a second peptide consisting of the amino acid sequence of SEQ ID NO:
- 4, sucrose, and sodium chloride in a solution of pH 12.0 to pH 13.0;
- b) adjusting the pH of the solution prepared by said step a) to pH 11.0 to pH 12.0 with an acid substance;
- c) adding a third peptide consisting of the amino acid sequence of SEQ ID NO:
- 2 and a fourth peptide consisting of the amino acid sequence of SEQ ID NO:
- 3 to the solution prepared by said step b);
- d) adjusting the pH of the solution prepared by said step c) to pH 9.0 to pH 11.0 with an acid substance;
- e) adding a fifth peptide consisting of the amino acid sequence of SEQ ID NO:
- 5 to the solution prepared by said step d);
- f) adjusting the solution prepared by said step e) to pH 7.0 to pH 9.0 with an acid substance;
- and
- g) lyophilizing the mixturesolution prepared by said step f).
Dependencies are inferred from the claim text.