Technical method for treating vitiligo through hair follicle melanocyte stem cell transplantation

An in-vitro preparation method of hair follicle melanocyte stem cells comprising the following steps:

• step S1:
• extracting one unit of hair follicles by using a vitiligo hair follicle extractor, wherein colored single-hair follicles or double-hair follicles are firstly selected, and the hair follicles with white hairs are not selected and meanwhile, 5-60 ml of blood are drawn from patients and then centrifugally separated to obtain required serum for in-vitro culture;
• step S2, separating a single complete outer hair root sheath with epidermis and containing hair follicle melanocyte stem cells from the hair follicles under a 2-15× magnifying glass or electron microscope, wherein the outer single complete hair root sheath contains all the melanocyte stem cells and mature melanocytes extracted from the hair follicles;
• step S3, putting the single-hair follicle of the complete outer hair root sheath containing the hair follicle melanocyte stem cells into a hair follicle storage ice box for culture, to enhance the activity of the hair follicle melanocyte stem cells and promote the differentiation and proliferation of the hair follicle melanocyte stem cells and transformation into the mature melanocytes, and culturing the mature melanocytes into a special culture solution at 0-4° C. for 60 min, and irradiating the cultured melanocytes for 5-50 s with 308NM excimer laser for later use wherein, the main ingredients of the special culture solution are psoralea corylifolia injection, dexamethasone sodium phosphate injection, autologous serum, low-molecular-weight heparin sodium and normal saline;
• and
• step S4, inactivating the hair follicles with the help of a dermoscope and a novel vitiligo hair follicle inactivation needle.