Rapid verification of virus particle production for a personalized vaccine

A method of producing a neoantigen vector for use in generating a neoantigen vaccine for a subject in need thereof, the method comprising:

• transfecting a plasmid into adenoviral production cells, wherein the plasmid comprises (1) a polynucleotide encoding adenoviral proteins and (2) a polynucleotide insert encoding at least one tumor-derived neoantigen, thereby producing and propagating adenoviral vectors in the adenoviral production cells;
• lysing the adenoviral production cells, wherein the lysate comprises the adenoviral vectors and adenovirus-associated hexon protein;
• loading the lysate onto an optical biosensor comprising a glass surface and an anti-hexon monoclonal antibody;
• measuring biomass accumulation on the glass surface by bio-layer interferometry (BLI), wherein the accumulation correlates with adenovirus-producing hexon positive production cells;
• quantifying hexon positive production cells, wherein quantification of the purified lysate by BLI occurs within 5-30 minutes, and is correlated with infectivity of the adenoviral vector;
• and thereby confirming production of infective adenoviral vectors for further propagation in a suspension cell line.