Compositions and methods for measuring and inhibiting calpain-5 activity
AssigneesThe Board of Trustees of the Leland Stanford Junior University, University of Iowa Research Foundation, The United States Government as represented by the Department of Veterans Affairs
InventorsVinit Mahajan, Gabriel Velez, Young Joo Sun, Kellie A Schaefer, Alexander G Bassuk
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Abstract
Compositions, methods, and kits are provided for assaying calpain-5 activity and inhibition. In particular, novel peptide substrates are provided for detecting calpain-5, measuring calpain-5 activity, and screening for inhibitors of calpain-5 to identify potential therapeutic agents for treating retinal diseases and other diseases associated with calpain-5 hyperactivity. Additionally, novel inhibitors of calpain-5 are also provided.
Claims5
A calpain-5 inhibitor selected from the group consisting of:
- a) a calpain-5 inhibitor comprising the amino acid sequence of SEQ ID NO:288 or SEQ ID NO:289, wherein the calpain-5 inhibitor comprises a lysine having a methylated epsilon amino group at position 7 of SEQ ID NO:288, or the calpain-5 inhibitor comprises a lysine having a methylated epsilon amino group at position 7 and position 8 of SEQ ID NO:289;
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- b) a calpain-5 inhibitor comprising an amino acid sequence having at least 90% identity to the sequence of SEQ ID NO:288 or SEQ ID NO:289, wherein the calpain-5 inhibitor comprises a lysine having a methylated epsilon amino group at position 7 of SEQ ID NO:288, or the calpain-5 inhibitor comprises a lysine having a methylated epsilon amino group at position 7 and position 8 of SEQ ID NO:289.
Dependencies are inferred from the claim text.