Modulation of carbon flux through the meg and C3 pathways for the improved production of monoethylene glycol and C3 compounds
AssigneeBraskem S.A.
InventorsAne Fernanda Beraldi Zeidler, Beatriz Leite Magalhaes, Lucas Pedersen Parizzi, Veronica Maria Rodege Gogola Kolling
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Abstract
The present disclosure provides methods of modulating the flux of carbon through the monoethylene glycol (MEG) biosynthesis pathway and one or more C3 compound biosynthesis pathways by expressing enzymes that are essential for improving C3 compounds and modulating other genetic aspects of MEG and C3 compound biosynthesis. The disclosure is further drawn to modified microbes comprising the disrupted sequences and overexpressed sequences, and compositions thereof.
Claims15
A recombinant Escherichia coli capable of coproducing MEG and one or more C3 compounds, wherein the recombinant E. coli comprises:
- a disruption of endogenous E. coli pta and one or more of the following (i) to (iii):
- (i) a disruption of endogenous E. coli ackA,
- (ii) a disruption of endogenous E. coli arcA, and
- (iii) a disruption of endogenous E. coli pka;
- wherein the recombinant E. coli comprises a pathway for MEG production;
- and
- wherein one of the one or more C3 compounds is acetone and the recombinant E. coli comprises a pathway for acetone production.
Dependencies are inferred from the claim text.