US 12,486,498 B2 · L-arginine-glycine amidinotransferase and use thereof in the production of guanidinoacetic acid

Abstract

The present invention discloses an L-arginine-glycine amidinotransferase and use thereof in the production of guanidinoacetic acid. In the present invention, through combined multi-site amino acid mutation, a technical effect of significantly improved enzyme activity of the mutant AkAGATT225Q/A258P/L278K than that of the wild-type strain is achieved, providing an application value for large-scale production of guanidinoacetic acid in industry. When the L-arginine-glycine amidinotransferase mutant constructed in the present invention is used in the production of guanidinoacetic acid, by optimizing the conversion conditions, the yield of guanidinoacetic acid is up to 21.4 g/L and the conversion rate is 90.4%, after 24 hrs of reaction in a 1 L reaction system. Compared with the production of guanidinoacetic acid with the raw enzyme, the yield is increased by 49.6%.

Claims1

Dependent claims

An L-arginine-glycine amidinotransferase comprising all of SEQ ID NO:

4 except for
(i) a glutamine at the position corresponding to position 225 of the polypeptide of SEQ ID NO:
4;
(ii) a glutamine at the position corresponding to position 225 of the polypeptide of SEQ ID NO:
4, and a proline at the position corresponding to position 258 of the polypeptide of SEQ ID NO:
4, or
(iii) a glutamine at the position corresponding to position 225 of the polypeptide of SEQ ID NO:
4, a proline at the position corresponding to position 258 of the polypeptide of SEQ ID NO:
4, and a lysine at the position corresponding to position 278 of the polypeptide of SEQ ID NO:
4.

7 more elements

Dependencies are inferred from the claim text.

Facts

Application

18/415,827Filed 18 Jan 2024 · continuation

Priority

CN 202310271233.420 Mar 2023

Published

US 2024/0344038 A117 Oct 2024

Granted

2 Dec 2025

Term ends

Not estimatedThe source records don’t support an estimate.

Classification11 codes

C12N 9/1003
Transferases: transferring one-carbon groups
C12N 9/1096
Transferases: transferring nitrogenous groups
C12N 15/70
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression: vectors or expression systems…
C12P 13/04
Preparation of nitrogen-containing organic compounds: alpha- or beta- amino acids

L-arginine-glycine amidinotransferase and use thereof in the production of guanidinoacetic acid

Life of this patent

L-arginine-glycine amidinotransferase and use thereof in the production of guanidinoacetic acid

Life of this patent

Abstract

Claims1