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AssigneeYoujiang Medical University for Nationalities
InventorsYanqiang Huang, Zansong Huang, Aixing Guan, Ganrong Huang, Xiangkun Qin
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A culture medium for testing drug resistance of H. pylori as well as a preparation method and use thereof are characterized in that 10% to 15% of calf serum, 1.2 mg/mL to 2.4 mg/mL of urea, 0.004 mg/mL to 0.016 mg/mL of phenol red, 10 μmol/L to 100 μmol/L of nickel chloride and H. pylori selective additive accounting for 1% of the total volume of the culture medium are added on the basis of a Columbia culture medium, and meanwhile, antibiotics for testing drug resistance are added, and the pH value is adjusted to 7.15 to 7.35. The present invention provides a method for a rapid testing of H. pylori resistance in culture media.
A culture medium for testing drug resistance of H. pylori, wherein a composition of the culture medium is based on a Columbia culture medium, with an addition of calf serum accounting for 10 wt. % to 15 wt. % of the culture medium, 1.2 mg/mL to 2.4 mg/ml of urea, 0.004 mg/mL to 0.016 mg/mL of phenol red, 10 μmol/L to 100 μmol/L of nickel chloride and H. pylori selective additive (Dent, SR0147E) accounting for 1% of a total volume of the culture medium, and meanwhile antibiotics for testing drug resistance are added, and a pH value is adjusted to 7.15 to 7.35.
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