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AssigneeHangzhou Converd Co., Ltd.
InventorsYunfan Liu, Wenbo Wang, Liming Zhang, Qian Chen, Sanmao Kang
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The present invention provides a method for producing a virus and a harvesting solution composition. The method includes culturing cells, wherein the cells have been inoculated with viruses or have been transfected with viral packaging elements; and contacting the cultured cells with a harvesting solution composition to harvest the viruses in a single step, wherein the harvesting solution composition comprises a trypsin, a pH buffer, and, optionally, a nuclease, and wherein the pH of the harvesting solution composition is greater than 7.5 and no more than 10.5. The virus production method of the present invention has advantages of simple operation, easy scale-up, stable yield and so on, and the yield is unexpectedly and significantly improved compared to the prior art, and it can ensure the integrity of the viral particles without damaging the biological activity of the viruses. Therefore, it is very suitable for large-scale production of viruses.
A method of producing a virus, comprising:
A harvesting solution composition for harvesting viruses subjected to cell culture, wherein the harvesting solution composition comprises a trypsin, a pH buffer, and, optionally, a nuclease, and
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