Porous aerogel scaffold, method of preparing the same and use thereof

A method of three-dimensional (3D) cell culture comprising the following steps:

• adding a photoinitiator and polyethylene glycol diacrylate in a buffer solution, heating to dissolve and mix evenly, then adding Poloxamer 407 into the mixed solution, and leaving to stand at low temperature to obtain an aerogel scaffold material;
• and printing a hydrogel scaffold by using a 3D printing technology, and performing an ultraviolet (UV) irradiation on the printed hydrogel scaffold to generate a cross-linkage, then forming a structurally stable 3D scaffold, performing a low-temperature soaking to remove the Poloxamer 407, performing a freeze drying to obtain the porous aerogel scaffold;
• wherein, the photoinitiator is a lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP) photoinitiator;
• in the buffer solution, a concentration of the LAP photoinitiator is 0.1-0.25 wt %, a concentration of polyethylene glycol diacrylate is 10-15 wt %, and a concentration of the Poloxamer 407 is 15-25 wt %;
• performing the low-temperature soaking of the 3D scaffold by using phosphate buffered saline (PBS), to remove the Poloxamer 407;
• wherein a temperature of the low-temperature soaking is 0-5° C., a duration time of the low-temperature soaking is 20-30 h;
• and a temperature of the freeze drying is −60-−50° C., and a duration time of the freeze drying is 20-30 h;
• performing a soaking sterilization on the porous aerogel scaffold by using an ethanol solution, soaking the sterilized porous aerogel scaffold in a complete culture medium, incubating at 37° C., 5% CO2 for 20-30 h, carrying out a functionalization of the porous aerogel scaffold;
• and, adding a cell suspension solution to the functionalized porous aerogel scaffold and culturing in a cell incubator at 37° C. and 5% CO2.