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US 12,486,315 B2Granted
Developing an efficient hybridoma platform for therapeutic antibody discovery
AssigneeGenentech, Inc.
InventorsMeredith Carroll Hazen, Zhonghua Lin, Dhaya Seshasayee
Life of this patent
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Abstract
The instant technology generally relates to improved methods for producing antibodies, antibody libraries, hybridomas, hybridoma libraries, etc. For example, these methods increase the number of antigen-specific B cells produced, increase the number of hybridomas, and/or increase the number of monoclonal antibodies that can be made in a given production cycle.
Claims17
A method for producing an antibody library, the method comprising:
- (a) injecting one or more animals with an antigen;
- (b) harvesting draining lymph nodes comprising B cells from each animal;
- (c) forming a hybridoma between each B cell and a fusion partner;
- and
- (d) screening the hybridomas for IgG having binding specificity to the antigen;
- wherein at least five of the following conditions apply:
- (i) the animals are outbred animals;
- (ii) the animals are injected at multiple sites;
- (iii) the animals are injected every two weeks;
- (iv) the animals are injected for between 6 weeks and 15 weeks;
- (v) multiple adjuvants are used, such that different animals are injected with different adjuvants;
- and/or
- (vi) B cells are enriched prior to step (c).
10 more elements
Dependencies are inferred from the claim text.