Three resin reactors in series peptide synthesizer

A process for coupling amino acid “X” to a protected N-group that is attached to a peptide synthesis resin found in a first reactor and a second reactor, comprising:

• adding a first quantity of de-protecting reagent to the first reactor;
• removing the first quantity of de-protecting reagent from the first reactor
• adding the first quantity of de-protecting reagent to the second reactor;
• adding a second quantity of de-protecting reagent to the first reactor;
• removing the first quantity of de-protecting reagent from the second reactor;
• removing the second quantity of de-protecting reagent from the first reactor
• adding the second quantity of de-protecting reagent to the second reactor;
• removing the second quantity of de-protecting reagent from the second reactor;
• adding a first quantity amino acid “X” activated ester to the first reactor;
• removing the first quantity amino acid “X” activated ester from the first reactor;
• adding the first quantity of amino acid “X” activated ester to the second reactor;
• adding a second quantity of amino acid “X” activated ester to the first reactor;
• removing the first quantity of amino acid “X” activated ester from the second reactor;
• removing the second quantity of amino acid “X” activated ester from the first reactor;
• adding the second quantity of amino acid “X” activated ester to the second reactor;
• and
• removing the second quantity of amino acid “X” activated ester from the second reactor.

A process for coupling amino acid “X” to a de-protected N-group attached to a peptide synthesis resin comprising:

• obtaining a first reactor and a second reactor, the first reactor and the second reactor each containing a quantity of a de-protected N-group attached to a peptide synthesis resin;
• adding a first quantity amino acid “X” activated ester to the first reactor;
• removing the first quantity amino acid “X” activated ester from the first reactor;
• adding the first quantity of amino acid “X” activated ester to the second reactor,
• adding a second quantity of amino acid “X” activated ester to the first reactor;
• removing the first quantity of amino acid “X” activated ester from the second reactor;
• removing the second quantity of amino acid “X” activated ester from the first reactor;
• adding the second quantity of amino acid “X” activated ester to the second reactor;
• and
• removing the second quantity of amino acid “X” activated ester from the second reactor.

A process for de-protecting a protected N-group attached to a peptide synthesis resin comprising:

• obtaining a first reactor and a second reactor, the first reactor and the second reactor each containing a quantity of a protected N-group attached to a peptide synthesis resin;
• adding a first quantity of de-protecting reagent to the first reactor;
• removing the first quantity of de-protecting reagent from the first reactor
• adding the first quantity of de-protecting reagent to the second reactor;
• adding a second quantity of de-protecting reagent to the first reactor;
• removing the first quantity of de-protecting reagent from the second reactor;
• removing the second quantity of de-protecting reagent from the first reactor;
• adding the second quantity of de-protecting reagent to the second reactor;
• and
• removing the second quantity of de-protecting reagent from the second reactor.