Chimeric antigen receptor to which anti-cotinine antibody is linked, and use thereof

A method for inducing cell death of a chimeric antigen receptor cell in a subject, the method comprising:

• administering a cotinine-conjugated cytotoxic agent to the subject,
• wherein the subject carries the chimeric antigen receptor cell which is formed in the subject by way of a previous administration of a cotinine-conjugated binding molecule and a cell transduced with a virus comprising a nucleic acid molecule,
• wherein the nucleic acid molecule encodes a chimeric antigen receptor,
• wherein the chimeric antigen receptor comprises, in the order from the N-terminus to the C-terminus of the chimeric antigen receptor, the following (a)-(d):
• (a) an anti-cotinine single chain Fv (scFv) antibody, wherein the anti-cotinine scFv antibody comprises the amino acid sequence of SEQ ID NO:
• 1;
• (b) a CD8 (cluster of differentiation 8) hinge domain comprising the sequence of SEQ ID NO:
• 3;
• (c) a transmembrane domain of CD28 comprising the sequence of SEQ ID NO:
• 5 or 7;
• and
• (d) a signal transduction domain selected from the group consisting of the following (i)-(iii):
• (i) a CD28 cytoplasmic region comprising the sequence of SEQ ID NO:
• 9 or 11,
• (ii) a CD3 zeta cytoplasmic region comprising the sequence of SEQ ID NO:
• 13, and
• (iii) a CD28 cytoplasmic region comprising the sequence of SEQ ID NO:
• 9 or 11 and a CD3 zeta cytoplasmic region comprising the sequence of SEQ ID NO:
• 13,
• wherein cytotoxic agent of the cotinine-conjugated cytotoxic agent is duocarmycin, SN-38, calicheamicin, monomethyl auristatin E (MMAE), monomethyl auristatin F (MMAF), doxorubicin, pyrrolobenzodiazepine, DM4, or DM1, and
• wherein binding molecule of the cotinine-conjugated binding molecule is a peptide, a protein, a nucleic acid, or a chemical substance.