Safe bovine heparin, preparation method, and application

Safe bovine heparin (SB heparin) comprising a mixture of heparin molecules with less than 15% of disaccharides comprising 6-O-desulfated D-glucosamine [α1-4] 2-O-sulfated L-iduronic acid, wherein the SB heparin exhibits anticoagulant potency equal or superior to 180 heparin international units (IUs) per mg of heparin in assays of inactivation of active thrombin promoted by potentiation of antithrombin, and wherein the SB heparin is prepared by:

• (i) separating heterogeneous heparin molecules with different ionic charges, disaccharide compositions and anticoagulant activities present in unfractionated heparins obtained from bovine intestinal mucosa with ionic resins, through serial or progressive elution with aqueous solutions with different anionic strengths;
• (ii) obtaining the SB heparin fraction.