Low glucosinolate pennycress meal and methods of making

Pennycress plants comprising a loss-of-function mutation in least one endogenous pennycress gene encoding the polypeptide of SEQ ID NO:

• 6 or an allelic variant thereof, wherein said loss-of-function mutation reduces expression of said polypeptide and/or reduces enzymatic activity of the polypeptide of SEQ ID NO:
• 6 or said allelic variant thereof, wherein said allelic variant of SEQ ID NO:
• 6 has at least 99% sequence identity to SEQ ID NO:
• 6, and wherein a seed lot obtained from the plants exhibits a reduction in sinigrin content in comparison to the sinigrin content of a control seed lot which lacks said loss-of-function mutation.

A method for obtaining a progeny plant from a pennycress plant, wherein said pennycress plant comprises a loss-of-function mutation in at least one endogenous pennycress gene encoding the polypeptide of SEQ ID NO:

• 6 or an allelic variant thereof, wherein said loss-of-function mutation reduces expression of said polypeptide and/or reduces enzymatic activity of the polypeptide of SEQ ID NO:
• 6 or said allelic variant thereof, wherein said allelic variant of SEQ ID NO:
• 6 has at least 99% sequence identity to SEQ ID NO:
• 6, and wherein a seed lot obtained from said pennycress plant exhibits a reduction in sinigrin content in comparison to the sinigrin content of a control seed lot which lacks said loss-of-function mutation, said method comprising crossing said pennycress plant of to a pennycress plant with wild-type sinigrin levels, selfing a progeny plant of the cross, and selecting a progeny plant of the selfing that provides a seed lot that exhibits a reduction in sinigrin content in comparison to the sinigrin content of a control seed lot which lacks said loss-of-function mutation.