System of predicting sensitivity of Klebsiella against MeropeneM and method

A method of predicting sensitivity of Klebsiella against meropenem, comprising:

• obtaining a microbial sample from a patient with pneumonia;
• isolating a candidate Klebsiella strain from the microbial sample;
• obtaining copy numbers of a plurality of genes in the candidate Klebsiella strain;
• calculating a k value according to the formula
• k
• =
• -
• 1.44
• +
• 0.708
• ×
• (
• C
• ⁢
• 1
• -
• 0.223
• 0.757
• )
• -
• 0.66
• ×
• (
• C
• ⁢
• 2
• -
• 0.951
• 0.09
• )
• +
• 0.088
• ×
• (
• C
• ⁢
• 3
• -
• 0.952
• 0.103
• )
• +
• 3.048
• ×
• (
• C
• ⁢
• 4
• -
• 0.469
• 1.09
• )
• -
• 0.46
• ×
• (
• C
• ⁢
• 5
• -
• 0.196
• 0.67
• )
• ,
• wherein, in the formula:
• C1 is the number of mphA gene copies in the candidate Klebsiella strain,
• C2 is the number of marA gene copies in the candidate Klebsiella strain,
• C3 is the number of Klebsiella pneumoniae KpnE gene copies in the candidate Klebsiella strain,
• C4 is the number of KPC-1 gene copies in the candidate Klebsiella strain, and
• C5 is the number of floR gene copies in the candidate Klebsiella strain;
• calculating an Exp(−k) power value with e=2.718281828459045 as base and −k as exponent, wherein an Exp(−k) power value of <1 corresponds to a prediction that the candidate Klebsiella strain is sensitive to meropenem, and an Exp(−k) power value of ≥1 corresponds to a prediction that the candidate Klebsiella strain is resistant against meropenem;
• predicting that the candidate Klebsiella strain is sensitive to meropenem;
• and
• administering meropenem to the patient in a medication treatment to treat the pneumonia based on the prediction in response to the prediction that the candidate Klebsiella strain is sensitive to meropenem.