Method and illumination device of the adaptive optics in transmission or reflection microscopy

A method for optimizing parameters of a physical light propagation model, the method comprising:

• providing a physical model Mlight of a light propagation in an optical system;
• radiating an input light distribution Io using an illumination unit into an excitation path of the optical system;
• traversing the input light distribution Io through a scattering body, wherein the scattering body is arranged in the excitation path of the optical system and modifies the input light distribution Io to form a transmission light distribution ITR or to form a reflection light distribution IRE;
• recording the transmission light distribution ITR by a first camera or the reflection light distribution IRE by a second camera, each of the first camera and the second camera being connected to a computer unit;
• transferring the recorded transmission light distribution ITR or the recorded reflection light distribution IRE to the computer unit on which the physical model Mlight and an algorithm for calculating transmission distortion parameters VTR are stored so that a measurement of the first camera and a measurement of the second camera can each be transferred directly as an input to the algorithm;
• and
• computing the transmission distortion parameters VTR of the physical model Mlight based on the recorded transmission light distribution ITR of the first camera or the recorded reflection light distribution IRE of the second camera, wherein the transmission distortion parameters VTR characterize the scattering body,
• wherein,
• the physical light propagation model is a light propagation model in laser microscopy in a scanning method, and
• the light propagation in the optical system is a light propagation in a microscope.