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US 12,480,955 B2Granted
Deglycosylation methods for electrophoresis of glycosylated proteins
AssigneeRegeneron Pharmaceuticals, Inc.
InventorsYiming Zhao, Hunter Chen, Shao-Chun Wang, Timothy Riehlman, Gabriel Carreau, Ying Wang
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Abstract
The disclosure relates to methods of analyzing a post-translationally modified protein of interest using electrophoresis, the methods comprising deglycosylating the protein of interest after labeling.
Claims26
A method of analyzing a sample comprising a protein of interest, wherein the protein of interest comprises an Fc domain, the method comprising:
- a. denaturing the sample;
- b. labeling the denatured sample with a fluorescent label to produce a labeled sample;
- c. quenching un-reacted fluorescent label in the labeled sample;
- d. deglycosylating the labeled sample with an endoglycosidase;
- and
- e. performing electrophoresis on the labeled sample after deglycosylating;
- wherein the sample is denatured, labeled and quenched in steps (a) through (c) prior to deglycosylation in step (d).
4 more elements
Dependencies are inferred from the claim text.