Systems and methods for utilizing combined magnetic nanoparticles and nanobodies
OwnerJaime Heissinventor
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Abstract
Disclosed are systems, methods, and computer software for determining a conformational change in a structure of a protein. One method includes delivering a magnetic nanoparticle-nanobody (MNP-NB) complex to a sample containing a protein, where the MNP-NB complex will bind to the protein in the sample. An external magnetic field is applied to the sample with a magnetic field generation system. Signals are detected from the MNP-NB complex that reflect a response to the external magnetic field and a conformational change in a structure of the protein in the sample is determined from the signals.
Claims16
A method for determining a conformational change in a structure of a protein, the method comprising:
- delivering a magnetic nanoparticle-nanobody (MNP-NB) complex to a sample containing the protein, wherein the MNP-NB complex will bind to the protein in the sample;
- applying an external magnetic field with a magnetic field generation system to the sample;
- detecting signal from the MNP-NB complex that reflects a response to the external magnetic field, wherein the detecting comprises performing magnetic particle spectroscopy and/or AC susceptibility measurement;
- and
- determining the conformational change in the structure of the protein by detecting the signal in the sample and comparing the detected signal to a baseline signal obtained prior to the conformational change.
Dependencies are inferred from the claim text.