A temperature equalizing plate (1) for a micro plate reader (4), onto which temperature equalizing plate (1) a microplate (3) with wells (30;31;32) can be removably arranged, the temperature equalizing plate (1) comprising:
- a plate-shaped body extending in a first horizontal direction (X) and in a second horizontal direction (Y) and comprising four corner areas (10), four edge areas (11) and one center area (12), wherein each edge area (11) is arranged between two neighboring corner areas (10) and wherein the center area (12) is arranged between the four edge areas (11);
- and
- wherein a first vertical offset is provided between an upward facing corner surface (100) of the corner areas (10) and an upward facing edge surface (110) of the edge areas (11), and wherein a second vertical offset is provided between the upward facing edge surface (110) of the edge areas (11) and an upward facing center surface (120) of the center area (12), resulting in gaps (G1;G2;G3) between at least some of the upward facing surfaces (100;110;120) of the temperature equalizing plate (1) and corresponding wells (30;31;32) of the thereon arranged microplate (3).