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US 12,480,158 B1Granted
Methods and systems for processing polynucleotides
Assignee10x Genomics, Inc.
InventorsAlireza Salmanzadeh, Dagmar Walter
Life of this patent
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Abstract
The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.
Claims23
A method for analyzing a sample, comprising:
- (a) providing (i) a sample comprising a plurality of cells and extracellular ribonucleic acid (RNA) molecules and (ii) a plurality of binding agents each of which comprises a label of a plurality of labels, wherein the plurality of binding agents is configured to couple to the extracellular RNA molecules;
- (b) contacting the extracellular RNA molecules with the plurality of binding agents to generate a plurality of coupled extracellular RNA molecules;
- (c) detecting the plurality of coupled extracellular RNA molecules via the plurality of labels to determine an amount of the extracellular RNA molecules in the sample;
- (d) using the amount of the extracellular RNA molecules in the sample to determine if the plurality of cells in the sample is of sufficient quality for processing in a cell assay,
- wherein the cell assay is a nucleic acid analysis assay;
- and
- (e)
- (A) if the plurality of cells in the sample is determined, in (d), to be of sufficient quality for processing in the cell assay in (d), performing the cell assay with cells of the plurality of cells;
- or
- (B) if the plurality of cells in the sample is determined, in (d), to not be of sufficient quality for processing in the cell assay, withholding cells of the plurality of cells from the cell assay.
7 more elements
Dependencies are inferred from the claim text.