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US 12,480,119 B2Granted
RNA cleavage-induced transcript stabilizer and uses thereof
AssigneeMassachusetts Institute of Technology
InventorsRon Weiss, Breanna E. DiAndreth
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Abstract
Provided herein are genetic circuits and encoded RNA transcripts that produce an output molecule in response to an RNA cleavage event that removes a degradation signal. In some embodiments, the genetic circuits described herein may be used for detecting RNA cleaver activities (e.g., in a cell). Methods of using the genetic circuits described herein in diagnostic or therapeutic applications are also provided.
Claims19
A cleavage-induced transcript stabilizer comprising:
- (i) an RNA transcript comprising a ribonucleotide sequence encoding an output molecule followed, in order, by:
- an RNA stabilizer, a cleavage site for an RNA cleaver, and a degradation signal that leads to degradation of the RNA transcript;
- wherein the RNA cleaver is an endoribonuclease, a siRNA, a miRNA, or a ribozyme;
- and
- wherein the cleavage-induced transcript stabilizer comprises one or more recognition sites for an RNA repressor operably linked to the nucleotide sequence encoding the output molecule.
2 more elements
Dependencies are inferred from the claim text.